BLOOD COAGULATION AS STUDIED BY INTRAVENOUS INJECTION OF TISSUE EXTRACT

The effect of intravenous injection of trypsin inhibitor on the coagulation of blood.

Trypsin inhibitor.’ A crude preparation was obtained by the method described previously.2 Dialysis of the final preparation for twenty-four hours against saline in the ice-box was found to be necessary for the removal of a toxic factor present in the undialyzed material. Without dialysis, small amounts (o. -z cc.) of the material regularly killed the s-pound rabbits in a very short time (30 tO ...

Blood Plasma Proteins as Influenced by Intravenous Injection of Gum Acacia

By repeated weekly intravenous injections of gum acacia solution in dogs over periods of 4 to 5 months, it has been possible to maintain plasma protein concentration and total circulating protein at very low levels. If sufficient numbers of such injections are given and then discontinued, plasma protein concentration will remain below the normal limits for several more months. Acacia remains in...

The tissue factor requirement in blood coagulation.

Formation of thrombin is triggered when membrane-localized tissue factor (TF) is exposed to blood. In closed models of this process, thrombin formation displays an initiation phase (low rates of thrombin production cause platelet activation and fibrinogen clotting), a propagation phase (>95% of thrombin production occurs), and a termination phase (prothrombin activation ceases and free thrombin...

Termination of Pregnancy in the Rabbit by Intravenous Injection of Anterior Lobe Pituitary Extract

The phospholipid requirement of tissue factor in blood coagulation.

Using a coagulation assay specific for tissue factor, we found that removal of 95% of the tissue factor-phospholipid resulted in a loss of 98% of its biological activity. The activity could be restored, with yields in excess of 100% by combining the extracted tissue factor with either mixed brain phospholipids or highly purified phospholipids. Phosphatidylethanolamine was the most active, follo...